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Easy Plate LS

Easy Plate LS is a custom media formulation for cultivation of Listeria species. A prepared plate that contains a chemical indicator that provides easily distinguishable blue-colored colonies, allowing you to easily identify and count Listeria species in 24±1 hours. Sold in packages of 100 tests in 4 separate bags containing 25 tests each.The shelf life of 18 months when stored unopened at 2-8℃.

Features

１． Easily distinguishable brightly colored colonies

Colonies turn blue, making them easy to visualize and count, even in the presence of food residue or other source of color.

Easy Plate LS	ALOA agar

Example: Listeria innocua (JCM 32814)

２． Excellent media performance

Faster Testing Enabled – Results equivalent to agar media in 24 hours.

Comparison of Performance : Easy Plate LS vs Plate Count Agar

Sample: Swabbed sample on artificially contaminated ceramic tile.
Swabbing solution: PBS

Strain: L. monocytogenes, Enterococcus faecalis

Plate Count Interpretation

Count all blue colonies regardless of size or intensity.
The appropriate number of colonies is between 1 and 250.

Example

Strains

Listeria monocytogenes (ATCC 19118)	Listeria monocytogenes (JCM 7671)	Listeria innocua (JCM 32814)

Listeria welshimeri (JCM 32816)	Listeria ivanovii (JCM 32891)	Listeria garayi (JCM 32813)

Listeria seeligeri (ATCC 51335)

Incubation conditions: 35℃, 24 hours (Diluent: phosphate buffered saline or buffered peptone water)
These colorings are for example only.

How to Use

For Quantitative Test


A1) Lift the cover and place 1 mL sample suspension onto the center of the plate.	A2) Lower the cover onto the sheet and allow the sample to spread evenly.	A3) Allow the plate to settle for 3 min or more on a horizontal surface for solidification.	A4) lncubate the plate(s) at 35±1℃ or 37±1℃ for 24 hours. Up to 25 plates can be stacked. After incubation, count all blue colonies regardless of size or intensity.	[Optional] Colony lsolation: Lift cover and pick a single colony form the gel.

For Qualitative Test


B1)Sample the selecvely enriched culture solution. For selective enrichment,please use Half-Fraser broth or similar selective enrichment media.	B2) Lift the cover and streak the selectively enriched culture onto the dry plate.	B3) Lift the cover and place 1 mL sterile water onto the center of the plate.	B4) Lower the cover onto the sheet and allow the sample to spread evenly.lncubate step is same as above step A4.	Listeria monocytogenes (JCM 7675)

* The sample preparation methods differ between environmental samples and food and beverage samples.
For the preparation methods applicable to each case, please refer to the instruction manual.

Product Specification

Product Name	Easy Plate LS
Product Code	61800
Object microorganism	Listeria species
Incubation Times	24 ± 1 hours
Incubation Temperature	35 ± 1℃ of 37 ± 1℃
Storage Condition (Unopened)	2 - 8℃
(After opening)	After opening the package, fold the open end of the package at least 2 times and tape it. Store in a refrigerator (2 to 8℃) and use within 3 months.
Shelf life	Shelf life 18 months after manufacturing date
Packaging	100 tests (25 tests x 4 bags)